Jurkat cells stably expressing human LILRB4 (Leukocyte Immunoglobulin-Like Receptor Subfamily B Member 4, GenBank Accession #NM_001278426) and the firefly luciferase gene under the control of NFAT response elements. Activation of NFAT in these cells can be monitored by measuring luciferase activity.

The expression of human LILRB4 was confirmed by flow cytometry analysis. The functionality of the cell line was validated using an anti-CD3 agonist antibody. Treatment of LILRB4-expressing NFAT-Luciferase reporter Jurkat cells with the anti-CD3 agonist antibody induced luciferase activity at a reduced level compared to the same treatment in the NFAT-Luciferase Reporter Jurkat cells (parental cells), consistent with the inhibitory function of LILRB4.

Illustration of the LILRB4 NFAT luciferase reporter Jurkat cell line.

In response to TCR activation, LILRB4 is tyrosine phosphorylated by Src-family kinases such as Lck or Fyn, which triggers recruitment of SH2 domain-containing phosphatases, leading to attenuation of the T cell activation signals.

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